On October 19, 1933, the National Emergency Committee called a General Mass Meeting at West New York, New Jersey, where by resolution; the Committee was authorized to call a General Convention of Delegates to be held in Yonkers, New York. Accordingly, the convention was (after two days of deliberation) adjourned to meet again on November 19, 1933, at New Britain, Connecticut, where the constitution and Bylaws were prepared and adopted. At this convention, the venerable Captain Alex Ameer of Yonkers was elected as the first president of the Federation.

The original associations represented at the first convention were the Assyrian National Union, Inc., of Massachusetts, the Assyrian National Association of Yonkers, New York, the Assyrian National Association of Connecticut, the Assyrian Christian Aid Society of Philadelphia, Pa., the Newark Branch of New Jersey and the Emergency Committee of West New York, New Jersey.

In 1958, the name of the Federation was changed to the Assyrian American Federation, Inc. and in 1962 the Federation was incorporated in the state of New York as the Assyrian American Federation, Inc. Affiliated with the Assyrian Universal Alliance in 1968. In 1982, the present name of the Assyrian American National Federation, Inc. was adopted.